Journal: Neurotoxicology and teratology

Article Title: Dose-Response Cocaine Pharmacokinetics and Metabolite Profile Following Intravenous Administration and Arterial Sampling in Unanesthetized, Freely Moving Male Rats

doi:

Figure Lengend Snippet: Concentration (mean ± SEM) vs. time curves for cocaine and major (BE) as well as minor metabolites (EME and NC) are shown for the animals that received an IV bolus injection of 3 mg/kg cocaine. The relative ordering of the metabolites indicated that BE was the primary metabolite, with average EME values approximately 1/10 that of BE and average NC values approximately 1/40 that of BE.

Article Snippet: Commercial standard solutions of cocaine metabolites at 1.0 mg/ml in methanol were from Alltech (State College, PA).

Techniques: Concentration Assay, Injection

Journal: Neurotoxicology and teratology

Article Title: Dose-Response Cocaine Pharmacokinetics and Metabolite Profile Following Intravenous Administration and Arterial Sampling in Unanesthetized, Freely Moving Male Rats

doi:

Figure Lengend Snippet: A dose-dependent shift in metabolite profile is illustrated as indexed by the area under the concentration (mean ± SEM) vs. time curve from T0 to T30. Although the AUC for the concentration vs. time function from T0 to T30 for each of the metabolites was similarly ordered for all dose groups (BE > EME > NC), a significant shift in metabolite concentrations was evident as a function of increasing cocaine dose. Whereas BE was increased, both EME and NC were depressed at the 3.0 mg/kg dose.

Article Snippet: Commercial standard solutions of cocaine metabolites at 1.0 mg/ml in methanol were from Alltech (State College, PA).

Techniques: Concentration Assay

Journal: Scientific Reports

Article Title: Sheathless CE-MS based metabolic profiling of kidney tissue section samples from a mouse model of Polycystic Kidney Disease

doi: 10.1038/s41598-018-37512-8

Figure Lengend Snippet: Comparison of base peak electropherograms of standard metabolite mixture consisting of 60 metabolites at 50 µM ( A ) and a QC sample of tissue sections ( B ) obtained with the sheathless CE-MS platform. Experimental conditions: BGE, 10% (v/v) acetic acid (pH 2.3); voltage, +20 kV; sample injection, 1 psi × 60 s (1.4% total volume of the capillary, i.e. roughly 9 nL); 91 cm × 30 µm i.d. × 150 µm o.d. fused-silica capillary.

Article Snippet: A standard metabolite mixture comprised of roughly 60 metabolites, with a concentration of 50 μM in water, was obtained from Human Metabolome Technologies (Tokyo, Japan), the so-called “HMT” mixture.

Techniques: Comparison, Injection

Journal: Journal of Personalized Medicine

Article Title: Clinical and Laboratory Associations with Methotrexate Metabolism Gene Polymorphisms in Rheumatoid Arthritis

doi: 10.3390/jpm10040149

Figure Lengend Snippet: rs4149081 and rs1476413 genotype associations. Statistically significant associations between two polymorphisms, plasma drug metabolite concentration, other laboratory and clinical outcome measures are shown for individual genotypes in methotrexate treated participants ( n = 66). Each symbol represents an individual participant of the genotype indicated on the x axes. Data grouped by rs4149081 genotypes: ( A ) baseline plasma concentration of unmetabolised methotrexate, ( B ) baseline plasma concentration of 7-hydroxy-methotrexate, ( C ) baseline bilirubin blood concentration, ( D ) weekly plasma concentrations (log scale) of listed methotrexate metabolites (PGs: polyglutamate subtypes) of a rs4149081 GA genotype participant. Data grouped by rs1476413 genotypes: ( E ) 6-week follow-up plasma concentration of 7-hydroxy-methotrexate, ( F ) baseline and ( G ) 6-week follow-up plasma concentrations of long-chain methotrexate 4-glutamate, ( H ) baseline and ( I ) 6-week follow-up disease activity (DAS28ESR) scores. Statistically significant differences between genotype group means are indicated by horizontal bars and an asterisk used to summarise p values adjusted by Bonferroni’s multiple comparison test: (*) p < 0.05; (**) p < 0.005 (descriptive statistics data shown in ). Red horizontal bar represents genotype group mean; error bars represent standard deviation. MTX: methotrexate; BL: baseline; 6w: 6-week follow-up.

Article Snippet: Control MTX metabolites and internal standards were from Schircks Laboratories (Jona, Switzerland).

Techniques: Clinical Proteomics, Concentration Assay, Activity Assay, Comparison, Standard Deviation

Journal: PLoS ONE

Article Title: Metabolic Footprint of Diabetes: A Multiplatform Metabolomics Study in an Epidemiological Setting

doi: 10.1371/journal.pone.0013953

Figure Lengend Snippet: The identity of the individual metabolites that are measured on each platform is provided in . Note that the metabolites metabolites that are quantified uniquely on the Biocrates platform carry specific information on the lipid side-chain composition of the different phospholipid classes (sometimes also referred to as lipidomics). The Metabolon platform, in contrast, provides a wider non-targeted, but semi-quantitative coverage of the general metabolome. NMR presently allows quantifying only a smaller set of metabolites, but this at a much higher degree of reproducibility, faster, and without specific sample preparation.

Article Snippet: In total, associations with 482 metabolites were tested (423 unique), with 201 metabolites from Biocrates, 257 from Metabolon, and 24 from Chenomx.

Techniques: Sample Prep

Journal: PLoS ONE

Article Title: Metabolic Footprint of Diabetes: A Multiplatform Metabolomics Study in an Epidemiological Setting

doi: 10.1371/journal.pone.0013953

Figure Lengend Snippet: List of selected metabolites that associate with diabetes at q-values <0.05 using log-scaled metabolite concentrations and assuming a linear model; %change is the increase or decrease of the mean in the diabetes group with respect to the control group; eta 2 is the proportion of the total variance that can be explained by the factor “diabetes” in the linear model; N is the number of valid data points that entered the analysis; the platform on which the corresponding metabolite was measured is indicated by the first letter of the provider: B = Biocrates, C = Chenomx, M = Metabolon.

Article Snippet: In total, associations with 482 metabolites were tested (423 unique), with 201 metabolites from Biocrates, 257 from Metabolon, and 24 from Chenomx.

Techniques: Control

Journal: PLoS ONE

Article Title: Metabolic Footprint of Diabetes: A Multiplatform Metabolomics Study in an Epidemiological Setting

doi: 10.1371/journal.pone.0013953

Figure Lengend Snippet: Difference of average branched chain amino acids (BCAA) concentrations between the diabetes and the control group; a positive value %-change indicates higher concentrations in the diabetes group; where metabolites that were measured on more than one platform, results from these platforms are presented separately.

Article Snippet: In total, associations with 482 metabolites were tested (423 unique), with 201 metabolites from Biocrates, 257 from Metabolon, and 24 from Chenomx.

Techniques: Control

Journal: PLoS ONE

Article Title: Metabolic Footprint of Diabetes: A Multiplatform Metabolomics Study in an Epidemiological Setting

doi: 10.1371/journal.pone.0013953

Figure Lengend Snippet: Overview of findings.

Article Snippet: In total, associations with 482 metabolites were tested (423 unique), with 201 metabolites from Biocrates, 257 from Metabolon, and 24 from Chenomx.

Techniques: Marker, Modification, Control, Activity Assay